Two photon versus one photon fluorescence excitation in whispering gallery mode microresonators

Year: 2016

Authors: Pastells C., Marco M.-P., Merino D., Loza-Alvarez P., Pasquardini L., Lunelli L., Pederzolli C., Daldosso N., Farnesi D., Berneschi S., Righini G.C., Quercioli F., Nunzi Conti G., Soria S.

Autors Affiliation: Nanobiotechnology for Diagnostics Group (Nb4Dg), IQAC-CSIC, Barcelona, 08034, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Barcelona, 08034, Spain; ICFO-Institut de Ciències Fotòniques, Castelldefels, Barcelona, 08860, Spain; Fondazione Bruno Kessler, Povo, TN, 38123, Italy; IBF-CNR, Povo, TN, 38123, Italy; Department of Computer Science, University of Verona, Strada le Grazie 15, Verona, 37134, Italy; CNR-IFAC Nello Carrara, Institute of Applied Physics, Sesto Fiorentino, FI, 50019, Italy; Museo Storico della Fisica e Centro Studi e Ricerche E. Fermi, Rome, 00184, Italy; CNR-INO National Institute of Optics, Sesto Fiorentino, FI, Italy

Abstract: We investigate the feasibility of both one photon and two photon fluorescence excitation using whispering gallery mode microresonators. We report the linear and non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a silica whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also investigate the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are dylight800, tetramethyl rhodamine isothiocyanate, rhodamine 6G and fluorescein. All measurements were performed in a modified confocal microscope.


Volume: 170      Pages from: 860  to: 865

More Information: S. Soria acknowledges CNR funding from the STM 2014 program. CNR-IFAC and IQAC-CSIC teams acknowledge funding from the CSIC-CNR bilateral projects. Funding from the Centro Fermi is gratefully acknowledged. Funding from the Progetto Premiale CNR-CF (2013–2015) is acknowledged. Authors also acknowledge the Laser Lab Europe (grant agreement no. 284464, EC\’s Seventh Framework Programme). This research has been partially supported by Fundació Cellex Barcelona and has been partially conducted at the super resolution light microscopy and nanoscopy facility (SLN) at ICFO. D. Farnesi is a PhD student at the University of Parma. F. Baldini and A. Giannetti are gratefully acknowledged for their support and discussions.
KeyWords: Fluorescence; Microspheres; Optical resonators; Photons; Resonators, Covalently bonded; Micro resonators; Non linear; Nonlinear enhancement; One-photon fluorescence; Real-time detection; Two photon fluorescence; Whispering gallery mode resonator, Whispering gallery modes
DOI: 10.1016/j.jlumin.2015.07.013

Citations: 4
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