Three-dimensional mapping of corneal lamellar orientation by means of backward-scattered SHG microscopy

Year: 2017

Authors: Mercatelli R., Ratto F., Rossi F., Menabuoni L., Malandrini A., Tatini F., Nicoletti R., Pini R., Pavone F.S., Cicchi R.

Autors Affiliation: National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara 1, Sesto Fiorentino, 50019, , Italy; Institute of Applied Physics N. Carrara (IFAC-CNR), Via Madonna del Piano 10, Sesto Fiorentino, 50019, , Italy; U.O. Oculistica Nuovo Ospedale S. Stefano, Via Suor Niccolina Infermiera 20, Prato, 59100, , Italy; C.S.O S.r.l, Via degli Stagnacci 12/e, Scandicci, 50018, , Italy; European Laboratory for Non-Linear Spectroscopy (LENS), Via Nello Carrara 1, Sesto Fiorentino, 50019, , Italy; Department of Physics, University of Florence, Via G. Sansone 1, Sesto Fiorentino, 50019, , Italy

Abstract: SHG microscopy was successfully used to characterize the orientation of sutural lamellae within corneal samples by means of an epi-detection scheme. Sutural lamellae are located in the first 30 µm of the stromal layer below Bowman

Conference title:

KeyWords: Image acquisition; Microscopic examination, Backward detections; Bowman’s membrane; Detection scheme; Lamellar orientations; Reduced stiffness; Three dimensional mapping; Three-dimensional correlation; Treatment follow-up, Stiffness

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