Clearing of fixed tissue: a review from a microscopist´s perspective

Year: 2016

Authors: Silvestri L., Costantini I., Sacconi L., Pavone F.S.

Autors Affiliation: National Institute of Optics, National Research Council, Via Nello Carrara 1, Sesto Fiorentino, Florence, 50019, Italy; European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, Sesto Fiorentino, Florence, 50019, Italy; University of Florence, Department of Physics and Astronomy, Via Sansone 1, Sesto Fiorentino, Florence, 50019, Italy

Abstract: Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this field has both triggered and been stimulated by recent advances in high-throughput microscopy and data analysis methods, which allowed imaging and management of large samples. The strong entanglement between clearing methods and imaging technology is often overlooked, as typical classification of the former is based only on the chemicals used. Here, we review the recent literature in the field, proposing a taxonomy of clearing techniques based on their mating with the major high-throughput microscopies. We hope that this application-oriented classification can help researchers to find the protocol best suited to their experiment among the many present in the literature. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)


Volume: 21      Pages from: 081205-1  to: 081205-8

More Information: The authors received funding support from the European Union Seventh Framework Program (FP7/2007-2013) under Grant No. 604102 (Human Brain Project) and Grant No. 284464 (LASERLAB-EUROPE). They have also been supported by the Italian Ministry for Education, University, and Research in the framework of the Flagship Project NanoMAX, and by \”Ente Cassa di Risparmio di Firenze\” (private foundation).
KeyWords: Imaging techniques; Throughput, Application-oriented; Data analysis methods; High throughput; Imaging technology; Light-sheet microscopies; Three-dimensional reconstruction; Tissue transformation; Two photon microscopy, Tissue, human; mating; microscopy; scientist; taxonomy; animal; brain chemistry; confocal microscopy; immunohistochemistry; mouse; procedures; staining; tissue fixation, Animals; Brain Chemistry; Immunohistochemistry; Mice; Microscopy, Confocal; Staining and Labeling; Tissue Fixation
DOI: 10.1117/1.JBO.21.8.081205

Citations: 103
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