Human dermal fibroblasts HDFa can be used as an appropriate healthy control for PMMA nanoparticles-survivin molecular beacon cellular uptake studies

Year: 2015

Authors: Adinolfi, B; Pellegrino, M; Baldini, F

Autors Affiliation: CNR, Ist Fis Applicata Nello Carrara, I-50019 Sesto Fiorentino, Italy – Univ Pisa, Dipartimento Ric Traslaz & Nuove Tecnol Med & Chi, I-56127 Pisa, Italy

Abstract: Survivin is a member of the inhibitor of apoptosis protein family (IAPs); besides its inhibitory action on apoptosis, it is also involved in the regulation of cell division. The protein expression is up-regulated in several cancers, being involved in the tumor progression and evasion of apoptosis. In line with its physiological roles, it is expressed also in several healthy tissues. The high expression of survivin in cancer cells correlates to poor prognosis and resistance to chemotherapeutic treatment, thus making this protein an attractive target in anticancer therapy. The dual role of survivin in cells, regulation of cell division and inhibition of apoptosis, combined with controversial data concerning the expression in normal tissues, emphasize the need to have an appropriate healthy control for in vitro studies. Aim of this study is to highlight this problem and to clarify the experimental conditions in which HDFa fibroblasts represent a negative control for survivin mRNA expression while ensuring the NPs-MB uptake. In this paper, by using confocal microscopy analysis supported by RT-and real-time-PCR experiments studies, we showed that HDFa fibroblasts represent a healthy negative control for survivin mRNA expression, only at very low cell density or at confluence. At the same time, we demonstrated that HDFa at any cell density are able to internalize NPs-MB after 6 h of treatment. (C) 2014 Published by Elsevier Masson SAS.


Volume: 69      Pages from: 228  to: 232

More Information: This work was supported by the national flagship project Nanomax. The authors wish to thanks the group of G. Varchi for the kind provison of PMMA-NPs and A. Giannetti, S. Tombelli and C. Trono (IFAC-CNR, Florence, Italy) as well as C. Domenici (IFC-CNR, Pisa, Italy) for the fruitful discussion.
KeyWords: Fibroblasts HDFa; Healthy control; Survivin expression; Polymethylmetacrilate nanoparticles (PM; Molecular beacon (MB)
DOI: 10.1016/j.biopha.2014.12.003

Citations: 8
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