Piezoelectric Nanoparticle-Assisted Wireless Neuronal Stimulation

Year: 2015

Authors: Marino, A; Arai, S; Hou, YY; Sinibaldi, E; Pellegrino, M; Chang, YT; Mazzolai, B; Mattoli, V; Suzuki, M; Ciofani, G

Autors Affiliation: Ist Italiano Tecnol, Ctr MicrobioRobot, I-56025 Pisa, Italy; Scuola Super Sant Anna, Biorobot Inst, I-56025 Pisa, Italy; WASEDA Biosci Res Inst Singapore WABIOS, Singapore 138667, Singapore; Univ Pisa, Dipartimento Ric Traslaz & Nuove Tecnol Med & Chi, I-56126 Pisa, Italy; Natl Univ Singapore, Dept Chem, MedChem Program, Inst Life Sci, Singapore 117543, Singapore; ASTAR, Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Singapore 138667, Singapore; Waseda Univ, Org Univ Res Initiat, Shinjuku Ku, Tokyo 1620041, Japan

Abstract: Tetragonal barium titanate nanoparticles (BTNPs) have been exploited as nanotransducers owing to their piezoelectric properties, in order to provide indirect electrical stimulation to SH-SY5Y neuron-like cells. Following application of ultrasounds to cells treated with BTNPs, fluorescence imaging of ion dynamics revealed that the synergic stimulation is able to elicit a significant cellular response in terms of calcium and sodium fluxes; moreover, tests with appropriate blockers demonstrated that voltage-gated membrane channels are activated. The hypothesis of piezoelectric stimulation of neuron-like cells was supported by lack of cellular response in the presence of cubic nonpiezoelectric BTNPs, and further corroborated by a simple electroelastic model of a BTNP subjected to ultrasounds, according to which the generated voltage is compatible with the values required for the activation of voltage-sensitive channels.

Journal/Review: ACS NANO

Volume: 9 (7)      Pages from: 7678  to: 7689

KeyWords: barium titanate nanoparticles; ultrasounds; piezoelectricity; SH-SY5Y cells; calcium imaging
DOI: 10.1021/acsnano.5b03162

Citations: 205
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