Excimer Laser-produced Biodegradable Photopolymer Scaffolds Do Not Induce Immune Rejection In Vivo

Year: 2015

Authors: Farkas B., Zsedenyi A., Gyukity-Sebestyen E., Romano I., Nagy K., Diaspro A., Brandi F., Buzas K., Beke S.

Autors Affiliation: Department of Nanophysics, Istituto Italiano di Tecnologia (IIT), Via Morego 30,
16163 Genova, Italy
University of Szeged, Faculty of Dentistry, Tisza Lajos krt. 64. H-6720 Szeged, Hungary
Hungarian Academy of Sciences, Biological Research Centre, Temesvari krt. 62,
H-6726 Szeged, Hungary
Istituto Nazionale di Ottica, Via Moruzzi 1, 56124-Pisa, Italy

Abstract: Following our previous works of in-vitro tests, the biocompatibility of photopolymer scaffolds was tested against immune responses in vivo. Neither relevant immune reactions nor the rejection of implanted scaffolds was detected, being an essential step for in vivo implantation of excimer laser-prepared scaffolds. The scaffolds were fabricated by UV excimer laser photocuring at 308 nm. After two weeks of transplantation neither inflammatory response nor reactive immune activation was detected based on the chemokine and cytokine profile. As a sign of biodegradability of the scaffolds, we detected macrophage infiltration and phagocytosis of the biopolymer at the site of implantation. Our results suggest that poly(propylene fumarate) (PPF): diethyl fumarate (DEF) (7 : 3 w/w) scaffolds have appropriate properties for in vivo applications.


Volume: 10 (1)      Pages from: 11  to: 14

More Information: This work has received funding support from the European Union (FP7-NMP-2013-EU-China) grant agreement n.604263 (NEUROSCAFFOLDS) and the grant of The Hungarian Social Renewal Operational Program (TAMOP-4.2.2-A-11/1/KONV-2012-0025). The project is co-financed by the European Union and the European Social Fund.
KeyWords: excimer lasers; scaffold fabrication; biocompatibility; biodegradation; in vivo experiments
DOI: 10.2961/jlmn.2015.01.0002

Citations: 3
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