Multiphoton multifocal microscopy exploiting a diffractive optical element

Year: 2003

Authors: Sacconi L., Froner E., Antolini R., Taghizadeh M.R., Choudhury A., Pavone FS.

Autors Affiliation: University of Trento, Via Sommarive 14, 38050 Povo (Trento), Italy; Department of Physics, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK; European Laboratory for Non-linear Spectroscopy (LENS), Via Nello Carrara 1, 50019 Sesto Fiorentino (Florence), Italy; Department of Physics, University of Florence, via Sansone 1, 50019 Sesto Fiorentino (Florence), Italy; Istituto Nazionale per la Fisica della Materia, Sezione di Firenze, via Sansone 1, 50019 Sesto Fiorentino (Florence), Italy

Abstract: Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds. (C) 2003 Optical Society of America.

Journal/Review: OPTICS LETTERS

Volume: 28 (20)      Pages from: 1918  to: 1920

KeyWords: Imaging systems; Microscopy; Optoelectronics
DOI: 10.1364/OL.28.001918

Citations: 122
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