Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging

Year: 2003

Authors: Ferraro P., De Nicola S., Finizio A., Coppola G., Grilli S., Magro C., Pierattini G.

Autors Affiliation: Istituto per la Microelettronica e i Microsistemi (IMM) del CNR, Sez. di Napoli, Via Diocleziano 328, 80124, Napoli, Italy;
Istituto di Cibernetica “E. Caianiello” del CNR, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
Istituto Nazionale di Ottica Applicata, Sez. di Napoli, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy;
Si-based optoelectronics, Bio- & nano-systems Group, STMicroelectronics srl. Stradale Primosole 50, 95121 Catania, Italy

Abstract: An approach is proposed for removing the wave front curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optical parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures. (C) 2003 Optical Society of America.

Journal/Review: APPLIED OPTICS

Volume: 42 (11)      Pages from: 1938  to: 1946

KeyWords: 3-dimensional Microscopy; Reconstruction; Aberrations
DOI: 10.1364/AO.42.001938

ImpactFactor: 1.534
Citations: 392
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