Myosin V fluorescence imaging dataset for single-molecule localization and tracking
Year: 2019
Authors: Gardini L., Arbore C., Pavone FS., Capitanio, M
Autors Affiliation: European Lab Nonlinear Spect, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; CNR, Natl Inst Opt, Largo Fermi 6, I-50125 Florence, Italy; Univ Florence, Dept Phys & Astron, Via Sansone 1, I-50019 Sesto Fiorentino, Italy
Abstract: Myosin-5B is one of three members of the myosin-5 family of actin-based molecular motors fundamental in recycling endosome trafficking and collective actin network dynamics. Through single-molecule motility assays, we recently demonstrated that myosin-5B can proceed in 36-nm steps along actin filaments as single motor. By analyzing trajectories of single myosin-5B along actin filaments we showed that its velocity is dependent on ATP concentration, while its run length is independent on ATP concentration, as a landmark of processivity.Here, we share image stacks acquired under total internal reflection fluorescence (TIRF) microscopy and representative trajectories of single myosin-5B molecules labelled with Quantum Dots (QD-myo-5B) moving along actin filaments at different ATP concentrations (0.3-1000 mM). Localization of QD-myo-5B was performed with the PROOF software, which is freely available [1]. The data can be valuable for researchers interested in molecular motors motility, both from an experimental and modeling point of view, as well as to researchers developing single particle tracking algorithms. The data is related to the research article “Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level” Gardini et al., 2015. (c) 2019 The Authors. Published by Elsevier Inc.
Journal/Review: DATA IN BRIEF
Volume: 25 Pages from: 103973-1 to: 103973-4
More Information: This work was supported by the European Union?s Horizon 2020 research and innovation program under grant agreement no. 654148 Laserlab-Europe and by Ente Cassa di Risparmio di Firenze.KeyWords: Total internal reflection fluorescence (TIRF) microscopy; Myosin; Single molecule biophysicsDOI: 10.1016/j.dib.2019.103973Citations: 2data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-10-13References taken from IsiWeb of Knowledge: (subscribers only)Connecting to view paper tab on IsiWeb: Click hereConnecting to view citations from IsiWeb: Click here