Characterization of human carotid atherosclerotic tissues imaged by combining multiple multiphoton microscopy techniques

Year: 2017

Authors: Baria E., Cicchi R., Nesi G., Massi D., Pavone F.S.

Autors Affiliation: European Laboratory for Non-Linear Spectroscopy, Via Nello Carrara 1, Sesto Fiorentino, 50019, Italy; National Institute of Optics, National Research Council, Largo Enrico Fermi 6, Florence, 50125, Italy; Department of Surgery and Translational Medicine, University of Florence, Largo Brambilla 3, Florence, 50134, Italy; Department of Physics, University of Florence, Via Sansone 1, Sesto Fiorentino, 50019, Italy

Abstract: We combined Second Harmonic Generation, Two-Photon Fluorescence and Fluorescence Lifetime Imaging Microscopy for studying human carotid ex vivo tissue sections affected by atherosclerosis, resulting in the discrimination of different arterial regions within the plaques.

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KeyWords: Fluorescence; Microscopic examination; Nonlinear optics, Ex-vivo; Fluorescence lifetime imaging microscopy; Multi-photon microscopy; Two photon fluorescence, Tissue