Neuroprotective Potential of Polyphenol-Rich Artichoke Waste Extract: Exploring Antioxidant and Immunomodulatory Activities in Cell Models
Year: 2025
Authors: Cuffaro D., Ceni C., Capitini C., Gabbia D., Zanotto I., Bertolini A., Mirabeni M., Bertini S., Saba A., De Martin S., Calamai M., Digiacomo M., Macchia M.
Autors Affiliation: Univ Pisa, Dept Pharm, Pisa, Italy; Univ Pisa, Interdept Res Ctr Nutraceut & Food Hlth, Pisa, Italy; Univ Florence, Sect Pharmaceut & Nutraceut Sci, Dept Neurosci Psychol Drug Res & Child Hlth NEUROF, Sesto Fiorentino, Italy; European Lab Nonlinear Spect LENS, Sesto Fiorentino, Italy; Univ Florence, Dept Phys & Astron, Sesto Fiorentino, Italy; Univ Padua, Dept Pharmaceut & Pharmacol Sci, Padua, Italy; Univ Pisa, Dept Surg Med & Mol Pathol & Crit Care Area, Pisa, Italy; Univ Siena, Dept Life Sci, Siena, Italy; Ctr Instrument Sharing Univ Pisa CISUP, Pisa, Italy; Natl Res Council Italy CNR, Natl Inst Opt, Florence, Italy.
Abstract: Artichoke waste (AW) constitutes a significant amount of discarded material, but it might be considered a sustainable source of phenolic compounds with antioxidant activity. This study aims to investigate the neuroprotective capacity of AW extract, due to its high content of polyphenolic compounds. In particular, the quali-quantitative characterization by HPLC and LC-MS/MS analysis revealed a notable presence of chlorogenic acid and flavonoids such as luteolin derivatives. The AW extract exhibited an antioxidant/antiradical potential demonstrated by its low IC50 values in DPPH and ABTS assays (0.14 and 0.073 mg/mL respectively). The AW extract was then evaluated in neuroinflammation processes on human neuroblastoma SH-SY5Y and THP-1 macrophage cell models, where it reduced the H2O2-induced oxidative stress and enhanced cell viability, by dropping the production of ROS at 25 mu g/mL. Furthermore, the AW ability in modulating inflammation was tested on THP-1-derived macrophages, demonstrating that the AW extract facilitated a shift in macrophage polarization from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype. These findings underscore the potential of AW extract for neuroprotection and inflammation modulation, highlighting the value of AW as a source of bioactive compounds and paving the route for further research into its mechanisms and clinical relevance. Furthermore, our results emphasize the significance of utilizing agricultural by-products.
Journal/Review: EFOOD
Volume: 6 (5) Pages from: e70094-1 to: e70094-14
More Information: This manuscript was funded by the Ministry of University and Research (MUR) as part of the PON 2014-2020Research and In-novationresources-Green/Innovation Action-DM MUR 1062/2021 Title of researchSviluppo di una piattaforma tecnologica per lo studio delle proprietanutraceutiche di biomolecule e biomateriali presenti negli scarti derivanti dalla filiera dei prodotti alimentari.KeyWords: antioxidant; artichoke waste; chlorogenic acid; neuroprotectionDOI: 10.1002/efd2.70094
