Force and torque measurements using magnetic micro beads for single molecule biophysics
Anno: 2003
Autori: Romano G., Sacconi L., Capitanio M., Pavone F.S.
Affiliazione autori: European Laboratory for Non-linear Spectroscopy, via Nello Carrara 1, 50019 Sesto Fiorentino, Florence, Italy; INFM sez.Firenze, via Sansone 1, 50019 Sesto Fiorentino, Florence, Italy Department of Physics, University of Florence, via Sansone 1, 50019 Sesto Fiorentino, Florence, Italy
Abstract: Characterization of optical trapping with laser tweezers of super-paramagnetic beads has been performed for the first time. In particular, we have compared the force action in optical trapping on super-paramagnetic beads with respect to the polystyrene ones. Using a novel magneto-optical manipulator we have developed a direct rotation detection scheme for trapped beads characterized by a high sensitivity and fast time response. Finally a calibration procedure able to directly measure an applied external torque on the trapped bead is shown. This demonstrates the capability of this apparatus to perform direct torque measurement (torsional rigidity) on a single bio-molecule attached to a super-paramagnetic bead. (C) 2002 Elsevier Science B.V. All rights reserved.
Giornale/Rivista: OPTICS COMMUNICATIONS
Volume: 215 (4-6) Da Pagina: 323 A: 331
Parole chiavi: Optical tweezers; Magnetic tweezers; Interferometric detection; Single molecule biophysics; Torque and force measurementsDOI: 10.1016/S0030-4018(02)02247-2Citazioni: 44dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2025-02-09Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui