Biosensing with a scanning planar Yagi-Uda antenna

Anno: 2022

Autori: Soltani N., Rabbany Esfahany E., Druzhinin S.I., Schulte G., Müller J., Butz B., Schönherr H., Agio M., Markešević N.

Affiliazione autori: Univ Siegen, Lab Nanoopt, D-57072 Siegen, Germany; Res Ctr Micro & Nanochem & Bio Technol C, D-57076 Siegen, Germany; Univ Siegen, Phys Chem 1, D-57076 Siegen, Germany; Univ Siegen, Micro & Nanoanalyt Grp, D-57076 Siegen, Germany; Natl Res Council CNR, Natl Inst Opt INO, I-50125 Florence, Italy; Univ Jyvaskyla, Nanosci Ctr, Jyvaskyla 40014, Finland.

Abstract: We investigate a model bioassay in a liquid environment using a z-scanning planar Yagi-Uda antenna, focusing on the fluorescence collection enhancement of ATTO-647N dye conjugated to DNA (deoxyribonucleic acid) molecules. The antenna changes the excitation and the decay rates and, more importantly, the emission pattern of ATTO-647N, resulting in a narrow emission angle (41°) and improved collection efficiency. We efficiently detect immobilized fluorescently-labeled DNA molecules, originating from solutions with DNA concentrations down to 1 nM. In practice, this corresponds to an ensemble of fewer than 10 ATTO-647N labeled DNA molecules in the focal area. Even though we use only one type of biomolecule and one immobilization technique to establish the procedure, our method is versatile and applicable to any immobilized, dye-labeled biomolecule in a transparent solid, air, or liquid environment.

Giornale/Rivista: BIOMEDICAL OPTICS EXPRESS

Volume: 13      Da Pagina: 539  A: 548

Maggiori informazioni: Deutsche Forschungsgemeinschaft (INST 221/118-1FUGG, INST 221/131-1); Bundesministerium fur Bildung und Forschung (13N14746); Universitat Siegen.
Parole chiavi: optical antennas, biosensors, planar antenna
DOI: 10.1364/BOE.445402

Citazioni: 1
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-09-08
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