Sliding of a single lac repressor protein along DNA is tuned by DNA sequence and molecular switching

Anno: 2018

Autori: Tempestini A., Monico C., Gardini L., Vanzi F., Pavone FS., Capitanio M.

Affiliazione autori: LENS European Laboratory for Non-Linear Spectroscopy; Universita degli Studi di Firenze; CNR – Istituto Nazionale di Ottica; International Center of Computational Neurophotonics

Abstract: In any living cell, genome maintenance is carried out by DNA-binding proteins that recognize specific sequences among a vast amount of DNA. This includes fundamental processes such as DNA replication, DNArepair, and gene expression and regulation. Here, we study the mechanism of DNA target search by a single lac repressor protein (LacI) with ultrafast force-clamp spectroscopy, a sub-millisecond and few base-pair resolution technique based on laser tweezers. We measure 1D-diffusion of proteins on DNA at physiological salt concentrations with 20 bp resolution and find that sliding of LacI along DNA is sequence dependent. We show that only allosterically activated LacI slides along non-specific DNA sequences during target search, whereas the inhibited conformation does not support sliding and weakly interacts with DNA. Moreover, we find that LacI undergoes a load-dependent conformational change when it switches between sliding and strong binding to the target sequence. Our data reveal how DNA sequence and molecular switching regulate LacI target search process and provide a comprehensive model of facilitated diffusion for LacI.


Volume: 46 (10)      Da Pagina: 5001  A: 5011

Maggiori informazioni: European Union´s Horizon 2020 Research and Innovation Programme [654148 Laserlab-Europe]; Italian Ministry of University and Research (FIRB ´Futuro in Ricerca´ 2013 [RBFR13V4M2], and Flagship Project NANOMAX); Ente Cassa di Risparmio di Firenze. Funding for open access charge: Italian Ministry of University and Research Flagship Project NANOMAX.
Parole chiavi: diffusion-driven mechanisms; facilitated target location; operator interaction; optical tweezers; nucleic-acids
DOI: 10.1093/nar/gky208

Citazioni: 15
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-05-19
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