Gephyrin oligomerization controls GlyR mobility and synaptic clustering

Anno: 2009

Autori: Calamai M., Specht CG., Heller J., Alcor D., Machado P., Vannier C., Triller A.

Affiliazione autori: INSERM U789 – Biologie Cellulaire de la Synapse 75005 Paris, France;
Departement de Biologie, Ecole Normale Superieure, 75005 Paris, France;
Institute of Biochemistry, University of Cologne, D-50674 Cologne, Germany

Abstract: High local concentrations of glycine receptors (GlyRs) at inhibitory postsynaptic sites are achieved through their binding to the scaffold protein gephyrin. The N- and C-terminal domains of gephyrin are believed to trimerize and dimerize, respectively, thus contributing to the formation of submembranous gephyrin clusters at synapses. GlyRs are associated with gephyrin also at extrasynaptic locations. We have investigated how gephyrin oligomerization influences GlyR dynamics and clustering in COS-7 cells and in cultured spinal cord neurons. To this aim, we have expressed isolated N- and C-terminal domains of gephyrin that interfere with the oligomerization of the full-length protein. We also studied the effect of an endogenous splice variant, ge(2,4,5), with a decreased propensity to trimerize. A reduction of the size and number of gephyrin-GlyR clusters was found in cells expressing the various interfering gephyrin constructs. Using fluorescence recovery after photobleaching, we studied the exchange kinetics of synaptic gephyrin clusters. Real-time single-particle tracking was used to analyze the mobility of GlyRs. We found that all the tested constructs displayed faster rates of recovery than wild-type gephyrin and increased the mobility of extrasynaptic receptors, showing that gephyrin-gephyrin interactions modulate the lateral diffusion of GlyRs. Furthermore, we observed an inverse correlation between GlyR diffusion properties and gephyrin cluster size that depended on the number of binding sites blocked by the different constructs. Since alterations in the oligomerization properties of gephyrin are related to the dynamics of GlyRs, the gephyrin splice variant ge(2,4,5) may be implicated in the modulation of synaptic strength.

Giornale/Rivista: JOURNAL OF NEUROSCIENCE

Volume: 29 (24)      Da Pagina: 7639  A: 7648

Maggiori informazioni: This work was supported by the Federation of European Biochemical Societies, the Association Francaise contre les Myopathies, and the Institut de Recherche sur la Moelle Epiniere. We thank C. Charrier and C. Ribrault for technical assistance.
Parole chiavi: gephyrin; glycine receptor, amino terminal sequence; animal cell; article; carboxy terminal sequence; controlled study; nonhuman; oligomerization; priority journal; protein expression; protein localization; protein protein interaction; rat; spinal cord nerve cell, Animals; Carrier Proteins; Cells, Cultured; Cercopithecus aethiops; COS Cells; Dendrites; Embryo, Mammalian; Fluorescence Recovery After Photobleaching; Luminescent Proteins; Membrane Proteins; Neurons; Protein Multimerization; Protein Structure, Quaternary; Protein Transport; Rats; Rats, Sprague-Dawley; Receptors, Glycine; Spinal Cord; Synapses; Time Factors; Transfection
DOI: 10.1523/JNEUROSCI.5711-08.2009

Citazioni: 71
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-05-19
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