Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy
Anno: 2011
Autori: Memmolo P., Di Caprio G., Distante C., Paturzo M., Puglisi R., Balduzzi D., Galli A., Coppola G., Ferraro P.
Affiliazione autori: Ist Nazl Ottica, CNR, I-80078 Pozzuoli, NA, Italy; Ist Nazl Ottica, CNR, I-73010 Arnesano, LE, Italy; Univ Naples Federico II, DIBET, I-80125 Naples, Italy; Ist Microelettron & Microsistemi, CNR, I-80131 Naples, Italy; Ist Sperimentale Italiano Lazzaro Spallanzani, I-26027 Rivolta Dadda, CR, Italy.
Abstract: An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques. Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data. (C) 2011 Optical Society of America
Giornale/Rivista: OPTICS EXPRESS
Volume: 19 (23) Da Pagina: 23215 A: 23226
Parole chiavi: In-line Holography; Reconstruction; Visualization; Spermatozoa; Fertility; Efficient; Binding; SpaceDOI: 10.1364/OE.19.023215Citazioni: 60dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2025-06-08Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui
