Surface charge and hydrodynamic coefficient measurements of Bacillus subtilis spore by optical tweezers
Anno: 2014
Autori: Pesce G., Rusciano G., Sasso A., Isticato R., Sirec T., Ricca E.
Affiliazione autori: Univ Naples Federico II, Complesso Univ Monte S Angelo, Dipartimento Fis, I-80126 Naples, Italy; CNR, Ist Nazl Ott, Sez Napoli, I-80078 Naples, Italy; Univ Naples Federico II, Complesso Univ Monte S Angelo, Dipartimento Biol, I-80126 Naples, Italy.
Abstract: In this work we report on the simultaneous measurement of the hydrodynamic coefficient and the electric charge of single Bacillus subtilis spores. The latter has great importance in protein binding to spores and in the adhesion of spores onto surfaces. The charge and the hydrodynamic coefficient were measured by an accurate procedure based on the analysis of the motion of single spores confined by an optical trap. The technique has been validated using charged spherical polystyrene beads. The excellent agreement of our results with the expected values demonstrates the quality of our procedure. We measured the charge of spores of B. subtilis purified from a wild type strain and from two isogenic mutants characterized by an altered spore surface. Our technique is able to discriminate the three spore types used, by their charge and by their hydrodynamic coefficient which is related to the hydrophobic properties of the spore surface.
Giornale/Rivista: COLLOIDS AND SURFACES B-BIOINTERFACES
Volume: 116 Da Pagina: 568 A: 575
Parole chiavi: B. subtilis; Spore; Electric charge; Hydrodynamic coefficient; Optical tweezersDOI: 10.1016/j.colsurfb.2014.01.039Citazioni: 33dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2025-05-18Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui
