Driving and analysis of micro-objects by digital holographic microscope in microfluidics
Anno: 2011
Autori: Merola F., Miccio L., Paturzo M., Finizio A., Grilli S., Ferraro P.
Affiliazione autori: CNR – Istituto Nazionale di Ottica, Via Campi Flegrei 34, 80078—Pozzuoli, Naples, Italy
Abstract: We propose an optical configuration in which floating particles in a microfluidic chamber can be characterized by an interference microscopy configuration to obtain quantitative phase-contrast maps. The configuration is simply made by two laser beams from the same laser source. One beam provides the optical forces for driving the particle along appropriate paths, but at same time works as the object illumination beam in the holographic microscope. The second beam plays the role of the reference beam, allowing recording of an interference fringe pattern (i.e., the digital hologram) in an out-of-focus image plane. The system and method are illustrated and experimental results are offered for polymeric particles as well as for in vitro cells with the aim to demonstrate the approach. (C) 2011 Optical Society of America
Giornale/Rivista: OPTICS LETTERS
Volume: 36 (16) Da Pagina: 3079 A: 3081
Parole chiavi: Quantitative Phase; Image-reconstruction; Refractive-index; Tracking; Chip; LensDOI: 10.1364/OL.36.003079Citazioni: 48dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2025-05-18Riferimenti tratti da Isi Web of Knowledge: (solo abbonati) Link per visualizzare la scheda su IsiWeb: Clicca quiLink per visualizzare la citazioni su IsiWeb: Clicca qui
