Dysplasia and tumor discrimination in brain tissues by combined fluorescence, Raman, and diffuse reflectance spectroscopies

Anno: 2023

Autori: Baria E., Giordano F., Guerrini R., Caporalini C., Buccoliero A.M., Cicchi R., Pavone F.S.

Affiliazione autori: CNR, Natl Inst Opt, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; Univ Florence, European Lab Nonlinear Spect, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy; A Meyer Childrens Hosp, Dept Neurosci 1, Div Neurosurg, Viale Gaetano Pieraccini 24, I-50141 Florence, Italy; Univ Florence, Dept Crit Care Med & Surg, Div Pathol, Viale Giovanni Battista Morgagni 85, I-50134 Florence, Italy; Univ Florence, Dept Phys & Astrophys, Via Sansone 1, I-50019 Sesto Fiorentino, Italy.

Abstract: Identification of neoplastic and dysplastic brain tissues is of paramount importance for improving the outcomes of neurosurgical procedures. This study explores the combined application of fluorescence, Raman and diffuse reflectance spectroscopies for the detection and classification of brain tumor and cortical dysplasia with a label-free modality. Multivariate analysis was performed to evaluate classification accuracies of these techniques–employed both in individual and multimodal configuration–obtaining high sensitivity and specificity. In particular, the proposed multimodal approach allowed discriminating tumor/dysplastic tissues against control tissue with 91%/86% sensitivity and 100%/100% specificity, respectively, whereas tumor from dysplastic tissues were discriminated with 89% sensitivity and 86% specificity. Hence, multimodal optical spectroscopy allows reliably differentiating these pathologies using a non-invasive, label-free approach that is faster than the gold standard technique and does not require any tissue processing, offering the potential for the clinical translation of the technology.

Giornale/Rivista: BIOMEDICAL OPTICS EXPRESS

Volume: 14 (3)      Da Pagina: 1256  A: 1275

Maggiori informazioni: Funding. Fondazione Cassa di Risparmio di Firenze (banking foundation; Human Brain Optical Mapping) ; Tuscany Region (projects DECODEE, GLIOMICS, research program PAR FAS 2007-2013) ; EU Horizon 2020 Res earch and Innovation Programme (grant 654148 Laserlab-Europe, grant 871277 AMPLITUDE) ; Italian Ministry for Education, University and Research (Flagship Project NANOMAX) .
Parole chiavi: Focal Cortical Dysplasia; Optical-properties; Label-free; Autofluorescence; Vivo; Neuroblastoma; Guidance
DOI: 10.1364/BOE.477035

Citazioni: 7
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2025-06-08
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