{"id":25048,"date":"2025-02-05T12:28:02","date_gmt":"2025-02-05T11:28:02","guid":{"rendered":"https:\/\/www.ino.cnr.it\/?p=25048"},"modified":"2025-02-05T12:28:02","modified_gmt":"2025-02-05T11:28:02","slug":"prin-2022-pnrr-the-restrimus-project","status":"publish","type":"post","link":"https:\/\/www.ino.cnr.it\/?p=25048","title":{"rendered":"PRIN 2022 PNRR | The ResTriMus project"},"content":{"rendered":"<h5><b>The project Regulation of striated muscle: a research bridging single molecule to organ &#8211; ResTriMus &#8211;<\/b> <span style=\"font-weight: 400\">is part of the PRIN2022 projects promoted by the MUR. It started on 01\/12\/2023 and will end on 30\/11\/2025.<\/span><\/h5>\n<p><span style=\"font-weight: 400\">The <\/span><span style=\"font-weight: 400\">ResTriMus<\/span> <span style=\"font-weight: 400\">project aims to describe the molecular mechanisms responsible for the regulation of contraction in the striated muscle and the related myopathies. In the sarcomere, the structural unit of the striated muscle, force and shortening are generated by two arrays of ATP-driven myosin motors. The sarcomere activity is controlled through a dual filament regulation: the calcium dependent structural change in the regulatory proteins on the thin filament, and the stress-dependent thick filament activation.\u00a0<\/span><\/p>\n<p><b>How Does <\/b><b>ResTriMus<\/b><b> Work?<\/b><\/p>\n<p><span style=\"font-weight: 400\">The <\/span><span style=\"font-weight: 400\">ResTriMus team<\/span><span style=\"font-weight: 400\"> employs advanced techniques to analyze normal, mutant, and engineered sarcomeric proteins both in situ and in vitro. It aims to define how thick filament regulation occurs in skeletal and cardiac muscles, focusing on how mutations in myosin and titin\u2014an essential cytoskeletal protein\u2014affect these processes. Additionally, it will explore cooperative thin filament activation mechanisms in cardiac muscle, particularly concerning mutations in the regulatory protein troponin.<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u00a0<\/span><b>Why Is It Important?<\/b><\/p>\n<p><span style=\"font-weight: 400\">The experiments conducted by ResTriMus project will serve to understand these molecular mechanisms that are crucial as mutations in regulatory proteins, and can lead to impaired filament activation, contributing to various myopathies and heart failure. By uncovering these pathways, the project aims to provide insights that could lead to new therapeutic strategies for treating these conditions.<\/span><\/p>\n<p><b>Project Goals:<\/b><\/p>\n<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Definition of thick filament regulation by the intramolecular and intermolecular interactions in myosin.\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Definition of the mechanokinetic parameters of the nanomachine powered by cardiac myosin.\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Implementation of a stochastic model that uses the nanomachine output to define the mechanism leading to myopathies, due to mutations in the myosin and in the cytoskeleton protein titin.\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Implementation of a multiscale model to describe the emergent properties of cooperativity from the half-sarcomere to the muscle cell.\u00a0<\/span><\/li>\n<\/ul>\n<p><b>Who is Participating?\u00a0<\/b><\/p>\n<p><span style=\"font-weight: 400\">The project involves a collaboration of experts from different institution: Prof. Marco Linari (University of Florence) as principal investigator and Dr. S. Gherardini (CNR-INO) as unit responsible.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u00a0<\/span><span style=\"font-weight: 400\">For more details about the project, you can contact <\/span><a href=\"mailto:stefano.gherardini@cnr.it\"><span style=\"font-weight: 400\">stefano.gherardini@cnr.it<\/span><\/a><span style=\"font-weight: 400\"> .<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><i><span style=\"font-weight: 400\">The project\u00a0 ResTriMus is funded by the European Union \u2013 Next Generation EU.<\/span><\/i><\/p>\n<p><i><span style=\"font-weight: 400\">Website PRIN:<\/span><\/i><a href=\"https:\/\/prin.mur.gov.it\/\"> <i><span style=\"font-weight: 400\">https:\/\/prin.mur.gov.it\/<\/span><\/i><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><i><span style=\"font-weight: 400\">Ph. Cr.: Adapted from Figure 1 in the reference &#8220;A mechanical model of the half-sarcomere which includes the contribution of titin&#8221; in Journal of Muscle Research and Cell Motility 40, pp. 29-41 (2019), by Irene Pertici, Marco Caremani, and Massimo Reconditi.<\/span><\/i><span style=\"font-weight: 400\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The project Regulation of striated muscle: a research bridging single molecule to organ &#8211; ResTriMus &#8211; is part of the PRIN2022 projects promoted by the MUR.<span class=\"excerpt-hellip\"> [\u2026]<\/span><\/p>\n","protected":false},"author":124061,"featured_media":25045,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[186,33],"tags":[238,470,1204],"class_list":["post-25048","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-homepage","category-researchfocus","tag-pnrr","tag-prin","tag-restrimus"],"_links":{"self":[{"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/25048","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/124061"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25048"}],"version-history":[{"count":2,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/25048\/revisions"}],"predecessor-version":[{"id":25050,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/25048\/revisions\/25050"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=\/wp\/v2\/media\/25045"}],"wp:attachment":[{"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25048"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25048"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ino.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25048"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}