Analysis of kinetics in noisy systems: Application to single molecule tethered particle motion

Anno: 2007

Autori: Vanzi F., Sacconi L., Pavone FS.

Affiliazione autori: LENS—European Laboratory for Nonlinear Spectroscopy, Sesto Fiorentino, Italy
Department of Biology and Animal Genetics “Leo Pardi”, University of Florence, Italy
Department of Physics, University of Florence, Italy

Abstract: In the tethered particle motion method the length of a DNA molecule is monitored by measuring the range of diffusion of a microsphere tethered to the surface of a microscope coverslip through the DNA molecule itself. Looping of DNA (induced by binding of a specific protein) can be detected with this method and the kinetics of the looping/unlooping processes can be measured at the single molecule level. The microsphere


Volume: 93 (1)      Da Pagina: 21  A: 36

Parole chiavi: tethered particle motion method
DOI: 10.1529/biophysj.106.094151

Citazioni: 12
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-05-19
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