Probing myosin structural conformation in vivo by second-harmonic generation microscopy

Anno: 2010

Autori: Nucciotti V., Stringari C., Sacconi L., Vanzi F., Fusi L., Linari M., Piazzesi G., Lombardi V., Pavone FS.

Affiliazione autori: Laboratory of Physiology, Department of Evolutionary Biology, University of Florence, 50125 Florence, Italy; European Laboratory for Non-Linear Spectroscopy, University of Florence, 50019 Sesto Fiorentino, Italy; Department of Physics, University of Trento, 38123 Povo, Italy; Department of Physics, University of Florence, 50019 Sesto Fiorentino, Italy

Abstract: Understanding of complex biological processes requires knowledge of molecular structures and measurement of their dynamics in vivo. The collective chemomechanical action of myosin molecules (the molecular motors) in the muscle sarcomere represents a paradigmatic example in this respect. Here, we describe a labelfree imaging method sensitive to protein conformation in vivo. We employed the order-based contrast enhancement by secondharmonic generation (SHG) for the functional imaging of muscle cells. We found that SHG polarization anisotropy (SPA) measurements report on the structural state of the actomyosin motors, with significant sensitivity to the conformation of myosin. In fact, each physiological/biochemical state we probed (relaxed, rigor, isometric contraction) produced a distinct value of polarization anisotropy. Employing a full reconstruction of the contributing elementary SHG emitters in the actomyosin motor array at atomic scale, we provide a molecular interpretation of the SPA measurements in terms of myosin conformations. We applied this method to the discrimination between attached and detached myosin heads in an isometrically contracting intact fiber. Our observations indicate that isometrically contracting muscle sustains its tetanic force by steady-state commitment of 30% of myosin heads. Applying SPA and molecular structure modeling to the imaging of unstained living tissues provides the basis for a generation of imaging and diagnostic tools capable of probing molecular structures and dynamics in vivo.


Volume: 107 (17)      Da Pagina: 7763  A: 7768

Maggiori informazioni: We thank Drs. C. Poggesi, C. Tesi, N. Piroddi, and D. Stampouli for preliminary experiments not included in this work. This research project has been supported by the European Community\’s Sixth Framework Program (Marie Curie Transfer of Knowledge Fellowship MTKD-CT-2004-BICAL-509761), by Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia, by Ministero dell\’Universita e della Ricerca, by the Ente Cassa di Risparmio di Firenze, and by Agenzia Spaziale Italiana project.
DOI: 10.1073/pnas.0914782107

Citazioni: 104
dati da “WEB OF SCIENCE” (of Thomson Reuters) aggiornati al: 2024-06-23
Riferimenti tratti da Isi Web of Knowledge: (solo abbonati)
Link per visualizzare la scheda su IsiWeb: Clicca qui
Link per visualizzare la citazioni su IsiWeb: Clicca qui